Tue, 2013-04-16 14:41 | 
The olive fruit fly, Bactrocera oleae, is the most important enemy of the olive. Its damaging effects make the insect a target of systematic control, mostly by using chemical insecticides. Beyond the harmful effects of pesticides to the environment, the excessive and irrational use has created a serious problem of insect resistance to the insecticides. In the past, there have been attempted without success environmentally friendly methods to control the populations of the flies, such as mass rearing and launching sterile insects in nature (SIT, Sterile Insect Technique). It has been shown that the existence of female insects at the time of launching makes difficult to succeed this method. However, the exemption of the female larvae in the mass rearing of the fly requires an in depth knowledge and the ability to manage the insect sex-determination pathways. For this purpose, we propose a basic transcriptomics and proteomics analysis for both male and female flies and also for susceptible and resistant to insecticides flies. The aim of this study is to identify and isolate genes which are involved in either sex-separation or resistance to insecticides pathways.
